Equipment available on PCBI platform
Quantification of bio-molecular interactions, by measuring the movement of molecules within a temperature gradient, by determining the KD.
Possible analyses:
- Molecular interaction YES/NO
- Determination of affinity (KD)
- Affinity screening
Benefits:
- Optimization/flexible protocol
- Feasible with different sample types (proteins, nucleic acids, small molecules, membrane and vesicles)
- Fast (KD obtained in 15min)
- Wide choice of stamp
- Detection of aggregates
- Wide affinity range (from pM to mM)
Study of the thermal stability of proteins and peptides thanks to their intrinsic fluorescence.
Possible analyses:
- Conformation quality control of proteins and peptides
- Protein-molecule interaction YES/NO
- Optimization of storage conditions
- Comparison of protein batches
Benefits:
- Fast (3min)
- Small quantity of samples (10μL)
- Labelling free
Complementary to bio-molecular analyses, this reader allows functional screening on cells of molecules identified as active compounds and the determination of IC50.
Possible analyses:
- FRET
- TR-FRET
- Fluorescence Polarization (FP)
- HTRF
- Luminescence
